NEWS RELEASE 6-NOV-2019
A game-changing test for Prion, Alzheimer's and Parkinson's diseases is on the horizon
Synthetic molecules made at Berkeley Lab can be used to diagnose numerous devastating illnesses
DOE/LAWRENCE BERKELEY NATIONAL LABORATORY
Enhanced detection of prion infectivity from blood by preanalytical enrichment with peptoid-conjugated beads
Simone Hornemann ,Petra Schwarz,Elisabeth J. Rushing,Michael D. Connolly,Ronald N. Zuckermann,Alice Y. Yam,Adriano Aguzzi Published: September 12, 2019https://doi.org/10.1371/journal.pone.0216013
Abstract
Prions cause transmissible infectious diseases in humans and animals and have been found to be transmissible by blood transfusion even in the presymptomatic stage. However, the concentration of prions in body fluids such as blood and urine is extremely low; therefore, direct diagnostic tests on such specimens often yield false-negative results. Quantitative preanalytical prion enrichment may significantly improve the sensitivity of prion assays by concentrating trace amounts of prions from large volumes of body fluids. Here, we show that beads conjugated to positively charged peptoids not only captured PrP aggregates from plasma of prion-infected hamsters, but also adsorbed prion infectivity in both the symptomatic and preclinical stages of the disease. Bead absorbed prion infectivity efficiently transmitted disease to transgenic indicator mice. We found that the readout of the peptoid-based misfolded protein assay (MPA) correlates closely with prion infectivity in vivo, thereby validating the MPA as a simple, quantitative, and sensitive surrogate indicator of the presence of prions. The reliable and sensitive detection of prions in plasma will enable a wide variety of applications in basic prion research and diagnostics.
FRIDAY, OCTOBER 25, 2019
27th ANNUAL REPORT 2018 CREUTZFELDT-JAKOB DISEASE SURVEILLANCE
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